The GitHub repos Hacker News keeps talking about
44,333 repositories ranked by 385,585 links in HN stories and comments since 2008.
44,333 repos · all time · by HN mentions
-
43351
A Python program to backup your simplenote.com
-
43352
Single-header C library for TCP/IPC messaging on Linux
-
43353
Easiest to use CLI to retrieve public files
-
43354
Tool to build your online resume
-
43355
FOSS clone of Everett Kaser's MESH:Hero puzzle game engine (git mirror of fossil repository)
-
43356
A fast, asynchronous, and easy-to-use TCP server library for the JVM (built on libuv).
-
43357
Self-hosted ngrok in one binary, with a TUI to inspect and replay every request.
-
43358
Run autoresearch on your Mac or any cloud GPU easily.
-
43359
Fun with prime numbers!
-
43360
A Resume built into a DOOM WAD
-
43361
create cardano or algorand proof-of-stake nodes at places like aws, digitalocean, vultr, linode, google, and more.
-
43362
Bitwig 6 working on Linux with Nix!
-
43363
Spend the subscription quota you didn't use and achieve your goals: finished, reviewable PRs in your own private repo.
-
43364
Firefox profile decryption tool and master password cracker.
-
43365
A simple yet powerful autocomplete jQuery plugin.
-
43366
JNU (Standing for as J is not Unix) is an open source monolithic kernel entirely vibe coded by AI.
-
43367
A list of popular and free Testnet Faucets (all easy, fast and safe to use) to get Testnet ETH and Tokens in 2025
-
43368
AI Telegram personal finance bot with cashflow forecasting and overdraft prediction
-
43369
A nice Optional template for Google Search Engine
-
43370
Library to read tracks raw data from audio CDs
-
43371
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
-
43372
Controlled chaos for your views, states, and screen sizes.
-
43373
OOptOut is now fPrivacy! See link.
-
43374
Catch missing and unused environment variables against your .env file
-
43375
Using LSA and related techniques to efficiently search the emails released in the Hacking Team data dump
-
43376
core concussionjs repository
-
43377
A real-time visualization layer for terminal-based interactive fiction, piping standard output into generative AI models.
-
43378
Crovia substrate — the 2026 archive: autonomous collectors, Ed25519-signed observation envelopes, batch Merkle seals, OpenTimestamps anchors, Phase-0 truth post-processors. Superseded for live observation by TACET (croviatrust/countersign).
-
43379
AI-powered PR description generator
-
43380
Stuff I write to learn Javascript
-
43381
The MEDICI Electronic Data Interchange Library
-
43382
JavaScript sandbox based on transpiler and shim
-
43383
Deprecated, see https://github.com/denysvitali/cv-2/ instead
-
43384
one image file that looks very different depending on your system
-
43385
Sample Use Cases of DevScore
-
43386
Style XML Feeds Without XSLT
-
43387
Securely sending query parameters in HTTP headers
-
43388
Firmware-agnostic compiler for 3D printer automation. Write once, deploy to Klipper, Marlin, RepRap, and more.
-
43389
Exploring tools for visualizing data with Python.
-
43390
Observer pattern in JS, implemented with promises.
-
43391
A data sharing service.
-
43392
-
43393
Easy to use library for working with core.matrix datasets in Clojure: select, where, aggregate, join, order, cross-tab, from/to-dataset, etc
-
43394
Lightweight PHP PDO wrapper for MySQL: CRUD helpers, prepared statements and a small query builder
-
43395
This demo shows how to use Farspeak (farspeak/farspeak-js) with Next.js server components.
-
43396
A minimal Mocha implementation for Node that's useful for REPL demos and RunKit
-
43397
Header-only ASCII font renderer for embedded/SDL/terminal
-
43398
Static site generator for my personal site, garrettgoss.com
-
43399
A tool to declaratively define and run stateful Docker containers for local web development.
- 43400